Bhawani Shankar Verma, Rachna khatri, Dr. Mohit Agrawal



Acinetobacter baumannii is one of the major pathogens causing nosocomial infections or healthcare-associated infection due to the emergence of resistance to various antimicrobial agents. Resistance due to antimicrobial degrading enzymes Like ESBL and MBLs is now a worldwide problem and a major reason for concern for the treating physicians.  

Materials and methods:

This is a cross-sectional study, a total of 55 Acinetobacter baumannii isolates from various clinical samples from November 2019 to April 2020. 

Acinetobacter baumannii isolates from various clinical samples were screened for Cephalosporins and Meropenem resistance for the detection of ESBL and MBL production. Carbapenemases production was confirmed by DDST and EDTA DST method for ESBLs and MBLs.


A total of 55 Acinetobacter baumannii isolates of in all clinical samples. Out of isolating Extended Spectrum Beta-Lactamases (ESBL) detection through screening method was 45 (81.82%) & confirmatory detection by DDST method was 42 (76.36%) and MBL (Metallo-β-Lactamases) detection by the screening method Carbapenems resistance were 40 (72.73%) and confirmatory detection EDTA DST method 35 (63.64%) respectively. Out of total isolates, most of the samples were Endotracheal Tube 35 (63.64%) & Blood 9 (16.36%).  Detection of ESBL by phenotypic method out of 45 (81.82%) samples resistance of 3rd generation Cephalosporins were Endotracheal aspirate 31 (56.36%) & blood 6 (10.91%) and 42 (76.37%) ESBL confirmatory detection by DDST method were 30 (54.54%) Endotracheal aspirate & 5 (9.09%) Blood respectively. Out of 40 (72.73%) MBL Positive by  Carbapenems resistance detection with Phenotypic Screening test Endotracheal aspirate 31 (56.36%) & Blood 5 (9.09%) and out of 35 (63.64%) Phenotypic Confirmatory by EDTA DST is Endotracheal Tube 30 (54.54%) & Blood 4 (7.27%) respectively.

 Acinetobacter baumannii isolates were 100% sensitive to Polymyxin B and Colistin for, confirmed MBL & ESBL production. Resistance to 100% Ceftazidime and Ceftriaxone for ESBL & MBL producer Acinetobacter baumannii confirmed ESBL and Meropenem and Imipenem both are antibiotics were 100% resistance in MBL producer. 


This study gives an alarming sign of a high ratio of multidrug resistance due to the production of extended-spectrum Beta-lactamase and Metallo beta Lactamases, respectively.


Acinetobacter, Carbapenems, MBL, ESBL, DST, and EDTA.

Full Text:



C. K. Murray and D. R. Hospenthal, “Treatment of multidrug resistant Acinetobacter,” Current Opinion in Infectious Diseases, vol. 18, no. 6, pp. 502–506, 2005.

M. L. Joly-Guillou, “Clinical impact and pathogenicity of Acinetobacter baumannii,” Clinical Microbiology and Infection, vol. 11, no. 11, pp. 868–873, 2005.

Peleg AY, Seifert H, Paterson DL. Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev. 2008;21(3):538–82.

Stephan C, Nathalie C, Eric E, Cecile G, Henri D, Alain R. AmpC cephalosporinase hyper production in clinical strains of Acinetobacter baumannii. J. Antimicrob. Chemother 2003; 52:629-35.

Higgins PG, Dammhayn C, Hackel M, Seifert H. Global spread of carbapenem-resistant Acinetobacter baumannii. J Antimicrob Chemother. 2010;65(2):233–8.

Sinha M, Srinivasa H. Mechanisms of resistance to carbapenems in meropenem- resistant Acinetobacter baumannii isolates from clinical samples. Indian J Med Microbiol. 2007;25(2):121–5.

Gupta V; An update on newer b-lactamases; Acinetobacter baumannii. Indian J Med Res, 2007;126: 417-27.

A. O. Okesola and A. A. Oni, “Occurrence of Extended-Spectrum Beta-Lactamase-Producing Pseudomonas aeruginosa and Acinetobacter baumannii Strains in South-West Nigeria,” Research Journal of Medical Sciences, vol. 6, no. 3, pp. 93–96, 2012.

Yan JJ, Wu JJ, Tsai SH, Chuang CL. Comparison of the double-disk, combined disk, and Etest methods for detecting metallo-beta-lactamases in gram-negative bacilli. Diagn Microbiol Infect Dis. 2004;49(1):5–11.

Clinical and Laboratory Standards Institute (2019) Performance standards for antimicrobial disk susceptibility tests; Approved standard- 29th edition. M02, A7 and M11 Clinical and Laboratory standard Institute, (2019), USA, 29(1): 1-76.

M. Banerjee, B. L. Chaudhary, and S. Shukla, “Prevalence of ESBL and MBL in Acinetobacter baumannii isolated from clinical samples in a tertiary care hospital,” Proceedings of the International Journal of Science and Research, vol. 4, no. 6, pp. 1183–1186, 2015.

Krista L, Epp S, Vivika A, Piert M, Lulu K, Karin O, et al. The prevalence and the antibiotic susceptibility of Acinetobacter baumannii, Pseudomonas aeruginosa and Klebsiella pneumoniae in Estonian intensive care units in comparison with European data. Scand J Infect Dis 2006; 38: 1001.

Amrit Koirala, Gaurav Agrahari, Nabaraj Dahal, Prakash Ghimire and Komal Raj Rijal ESBL and MBL mediated resistance in clinical isolates of non-fermenting gram negative bacilli (NFGNB) in Nepal Journal of Microbiology and Antimicrobial Agents. 2017; 3 (1): 18- 24.

Richa Hans, Dakshina Bisht, Ritu Agarwal And M.Irfan, phenotypic detection of mbl, ampc beta-lactamase and carbapenemases in multi drug resistant isolates of Acinetobacter baumannii int j med res health sci. 2015;4(2):311-316.

H. Kabbaj, M. Seffar, B. Belefquih “Prevalence of Metallo-𝛽-Lactamases Producing Acinetobacter baumannii in a Moroccan Hospital,”ISRN Infectious Diseases,vol.2013,Article ID154921,3pages,2013.

Hanoch S. I. Martins , Maria Rosa Q. Bomfim, Rafaela O. França1, Luiz M. Farias, Maria Resistance Markers and Genetic Diversity in Acinetobacter baumannii Strains Recovered from Nosocomial Bloodstream Infections ; Int. J. Environ. Res. Public Health 2014, 11, 1465-78.

Jaggi N. Acinetobacter baumannii isolates J Microbiol Infect Dis. 2012;2(2) June.

Muthusamy D, Appalaraju Boppe. Phenotypic methods for the detection of various beta lactamases in carbapenem resistant isolates of Acinetobacter baumanii at a tertiary care hospital in south India. Journal of Clinical and Diagnostic Research [serial online] 2012 ;6:970-73.

Amandeep Kaur and Satnam Singh Prevalence of Extended Spectrum Betalactamase (ESBL) and Metallobetalactamase (MBL) Producing Pseudomonas aeruginosa and Acinetobacter baumannii Isolated from Various Clinical Samples Hindawi Journal of Pathogens Volume 2018, Article ID 6845985, 7; 10.1155/2018/6845985.

Marzieh Safari, Amir Sasan. Mozaffari Nejad , Abas Bahador , Rasool Jafari , Mohammad Yousef Alikhani. Prevalence of ESBL and MBL encoding genes in Acinetobacter baumannii strains isolated from patients of intensive care units (ICU) Saudi Journal of Biological Sciences (2015) 22, 424–429.


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