COMPARISON OF ANTIMICROBIAL RESISTANCE PATTERN IN BIOFILM PRODUCER AND NON-BIOFILM PRODUCER ESCHERICHIA COLI FROM PATIENTS WITH UTI.

Rachna Khatri, Balamuruganvelu. S, Geethavani. B, Himanshu Tripathi

Abstract


Background: Escherichia coli (E.coli) is the most common isolated organism associated with Urinary tract infection(UTI). Escherichia coli form intracellular bacterial communities with biofilm-like properties within the bladder epithelium. Biofilm formation is one of the most important virulence factors exhibited by Escherichia coli among other virulence factors. Biofilm can restrict the diffusion of substances and the binding of antimicrobials enclosing them in an extracellular biochemical matrix. Objective: - The study was aimed to detect biofilm production by Uropathogenic Escherichia coli (UPEC) and to compare the antimicrobial resistance pattern of biofilm producing and non biofilm producing isolates from patients with UTI. Material & Methods: After obtaining institutional ethical approval, a total of 300 Uropathogenic Escherichia coli isolated from with UTI from July 2019 to December 2019 at the National Institute of Medical Science and Research (NIMS) Hospital, Jaipur, Rajasthan were tested for in vitro biofilm production by three different methods (Tube method, Congo red agar method and Tissue culture Plate method).  All isolates were tested for resistance to 15 different antibiotics by Kirby Bauer disc diffusion method. Result: Out of 300 Uropathogenic Escherichia coli isolates 55.7% of the isolates were found to be biofilm producers by TCP method, biofilm detection by TM showed to 62.7 of isolates with biofilm producers. Biofilm producing UPEC showed significantly higher degree of resistance to most/all of the antibiotics compare to Non Biofilm producing Uropathogenic Escherichia coli. However among biofilm producers low resistance was observed to few higher antibiotics such as Meropenem 24.6%, Imipenem 38.3%, Nitrofurantoin 41.3% and Piperacillin/Tazobactam 53.9%.

Conclusion: Biofilm producer Escherichia coli is responsible for high drugs resistance in comparison to non-biofilm producer.


Keywords


Biofilm, Resistance, E.coli

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